MIcroarray Facilities @ SCCP

Services & Pricing

  • Custom Arrays
  • Array Design
  • Hybridization
  • Scanning and Quantitation
  • Microarray Data Analysis
  • Analysis of RNA and DNA

Faculty Resources

  • Packard BioChip Arrayer
  • Packard Multiprobe II Liquid Handler
  • MAUI 4-Bay Hybridization System
  • Agilent High-Resolution Microarray Scanner
  • Agilent Hybridization Oven
  • Agilent 2100 Bioanalyzer
  • Microarray Links

Facility Staff

The Microarray Core Facility / Resources

Welcome to the Microarray Core Facility of the South Carolina College of Pharmacy (SCCP)

The Microarray Core Facility is located on the 6th floor of the Coker Life Science Building (Room 617) in the Department of Pharmaceutical and Biomedical Sciences at the University of South Carolina.

The DNA Microarray Facility receives partial support from SC INBRE, funded by the Institutional Development Award (IDeA) program of the National Center for Research Resources. Information about SC INBRE can be found at www.scinbre.org

The Facility is also a member of the Network of IDeA-funded Core Laboratories, (NICL). Find information about NICL and access network resources at www.niclweb.org

Agilent High-Res Microarray Scanner

Packard BioChip Arrayer

Packard Multiprobe II Liquid Handler

Packard_Multiprobe_II_Liquid_Handler

Agilent 2100 Bioanalyzer

Agilent 2100 Bioanalyzer

Agilent Hybridization Oven

Agilent Hybridization Oven at SCCP

4-Bay Hybridization System

4-Bay Hybridization System

Custom Arrays

The facility is well equipped to provide custom microarrays for academic and industrial clients. So far we have successfully spotted cDNAs, 50 to 70 base pair oligonucleotides and 18 to 22 base pair short primers. In addition to nucleic acids, the Biochip Arrayer can spot antibodies, proteins, peptides and bacterial cells as well. We produce arrays of the highest quality but at a low density (up to about 2,000 spots per slide).

Array Design

We assist all clients in designing the appropriate microarray for your needs. We take the guesswork out of array alignment and maintain a high level of spatial precision throughout the printing run. Specification with regard to array design and spot placement is tightly monitored. This attention to detail results in saved scanner time and fewer auto format failures.

Hybridization

We provide extensive on site training and support for all MAUI 4-bay Hybridization System and Agilent’s Hybridization Oven users.

Scanning and Quantitation

Support is provided for spotted microarray scanning with ScanAray Express SP3 and quantitative analysis with ScanAray Express SP3 and ImaGene software.

Microarray Data Analysis

We assist customers with gene expression data analysis using limma/limmaGUI and GeneSifter software. Additionally, we perform cluster analysis using Cluster and Treeview software both from Eisen laboratory.

Analysis of RNA and DNA

Quantity and quality of RNA and DNA are determined with the help of Agilent 2100 Bioanalyzer.